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A Reducing and Oxidizing System Minimizes Photobleaching and Blinking of Fluorescent Dyes

Authors

  • Jan Vogelsang,

    1. Angewandte Physik – Biophysik, and Center for NanoScience, Ludwig-Maximilians-Universität, Amalienstrasse 54, 80799 München (Germany), Fax: (+49) 89-2180-2050
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    • These authors contributed equally to this work.

  • Robert Kasper,

    1. Angew. Laserphysik and Laserspektroskopie, Universität Bielefeld, Universitätsstrasse 25, 33615 Bielefeld (Germany)
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    • These authors contributed equally to this work.

  • Christian Steinhauer,

    1. Angewandte Physik – Biophysik, and Center for NanoScience, Ludwig-Maximilians-Universität, Amalienstrasse 54, 80799 München (Germany), Fax: (+49) 89-2180-2050
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  • Britta Person Dr.,

    1. Angewandte Physik – Biophysik, and Center for NanoScience, Ludwig-Maximilians-Universität, Amalienstrasse 54, 80799 München (Germany), Fax: (+49) 89-2180-2050
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  • Mike Heilemann Dr.,

    1. Angew. Laserphysik and Laserspektroskopie, Universität Bielefeld, Universitätsstrasse 25, 33615 Bielefeld (Germany)
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  • Markus Sauer Prof. Dr.,

    1. Angew. Laserphysik and Laserspektroskopie, Universität Bielefeld, Universitätsstrasse 25, 33615 Bielefeld (Germany)
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  • Philip Tinnefeld Prof. Dr.

    1. Angewandte Physik – Biophysik, and Center for NanoScience, Ludwig-Maximilians-Universität, Amalienstrasse 54, 80799 München (Germany), Fax: (+49) 89-2180-2050
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  • We thank K. H. Drexhage for providing the oxazine derivative MR121 and C. Forthmann for help with data analysis. Support was provided by the DFG (SFB613), the Biophotonic III Program of the BMBF/VDI (Grant 13N9234), and the Nanosystems Initiative Munich (NIM).

Abstract

original image

On the ROXS: Photobleaching represents one of the main limitations of modern fluorescence microscopy. A reducing and oxidizing system (ROXS) has been introduced that recovers triplet states as well as charge-separated states through electron-transfer reactions (see picture). Thus the blinking and photobleaching of fluorophores is strikingly reduced.

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