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Combinatorial Synthesis of Peptide Arrays with a Laser Printer

Authors


  • We thank Dorothea Freidank, Daniela Rambow, Thorsten Kühlwein, and Jürgen Kretschmer for technical assistance, Martina Schnölzer (DKFZ) for mass spectrometry, Hans Lehrach for helpful discussions and encouragement, Kazuhiro Ohmori (Sekisui) for advice in particle composition, Alexander Küller, Michael Grunze, and Reiner Dahint (University of Heidelberg) for surface-analytical methods, and the BMBF (grant nos. 03N8710 and NGFN-0313375), the Helmholtz Society (grant no. VH-VI-108), the HFSPO (grant no. RGP5/2006), the European Union (grant no. 508399), and the Volkswagenstiftung (grant no. 79 466) for financial support.

Abstract

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Special delivery: The “freezing” of activated amino acid derivatives within solid particles enables a laser printer to deliver these “postal packages” to defined locations on a solid support with high resolution. Subsequent parallel coupling is initiated simply by melting a whole layer of 20 different amino acid particles (see schematic representation; Fmoc=9-fluorenylmethoxycarbonyl).

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