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Chemical Strategies for Generating Protein Biochips

Authors

  • Pascal Jonkheijm Dr.,

    1. Department of Chemical Biology, Max Planck Institute of Molecular Physiology and Faculty of Chemistry, Chemical Biology, Technical University of Dortmund, Otto Hahn Strasse 11, 44227 Dortmund (Germany), Fax: (+49) 231-133-2499
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  • Dirk Weinrich Dipl.-Chem.,

    1. Department of Chemical Biology, Max Planck Institute of Molecular Physiology and Faculty of Chemistry, Chemical Biology, Technical University of Dortmund, Otto Hahn Strasse 11, 44227 Dortmund (Germany), Fax: (+49) 231-133-2499
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  • Hendrik Schröder Dr.,

    1. Faculty of Chemistry, Biological–Chemical Microstructuring, Technical University of Dortmund, Otto Hahn Str. 6, 44227 Dortmund (Germany), Fax: (+49) 231-755-7082
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  • Christof M. Niemeyer Prof. Dr.,

    1. Faculty of Chemistry, Biological–Chemical Microstructuring, Technical University of Dortmund, Otto Hahn Str. 6, 44227 Dortmund (Germany), Fax: (+49) 231-755-7082
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  • Herbert Waldmann Prof. Dr.

    1. Department of Chemical Biology, Max Planck Institute of Molecular Physiology and Faculty of Chemistry, Chemical Biology, Technical University of Dortmund, Otto Hahn Strasse 11, 44227 Dortmund (Germany), Fax: (+49) 231-133-2499
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Abstract

Protein biochips are at the heart of many medical and bioanalytical applications. Increasing interest has been focused on surface activation and subsequent functionalization strategies for immobilizing these biomolecules. Different approaches using covalent and noncovalent chemistry are reviewed; particular emphasis is placed on the chemical specificity of protein attachment and on retention of protein function. Strategies for creating protein patterns (as opposed to protein arrays) are also outlined. An outlook on promising and challenging future directions for protein biochip research and applications is also offered.

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