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Semisynthesis of a Glycosylphosphatidylinositol-Anchored Prion Protein


  • Financial support from the Max Planck Society, the Deutsche Forschungsgemeinschaft, the Fonds der chemischen Industrie, the Swiss National Science Foundation (SNF grant 205321-107651), and ETH Zürich is gratefully acknowledged. The authors thank J. Tatzelt and M. Engelhard for helpful discussions.


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Pinning down the role of the anchor: The chemical synthesis of a cysteine-modified glycosylphosphatidylinositol (GPI) anchor provides access to homogeneous GPI-anchored prion protein through expressed protein ligation (see scheme). By this method, it should be possible to investigate the influence of the complex posttranslational GPI modification on protein structure and function.