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Keywords:

  • fluorescent probes;
  • imaging agents;
  • localization microscopy;
  • optical switches;
  • subdiffraction resolution
Thumbnail image of graphical abstract

Eagle eyes: dSTORM uses conventional photoswitchable fluorescent dyes that can be reversibly cycled between a fluorescent and a dark state by irradiation with light of different wavelengths (see picture). This elegant approach can visualize cellular structures with a resolution of approximately 20 nm, far beyond the diffraction limit of light, without the need of an activator molecule.