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Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes


  • The authors thank K. H. Drexhage for stimulating discussion and delivery of Alexa 647–phalloidin and Dr. G. Wiebusch for technical assistance. This work was supported by the Biophotonics program of the German Ministry of Research and Education.


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Eagle eyes: dSTORM uses conventional photoswitchable fluorescent dyes that can be reversibly cycled between a fluorescent and a dark state by irradiation with light of different wavelengths (see picture). This elegant approach can visualize cellular structures with a resolution of approximately 20 nm, far beyond the diffraction limit of light, without the need of an activator molecule.

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