The work was supported by the DFG (SFB 498, Cluster of Excellence “Unicat”).
Monitoring Catalysis of the Membrane-Bound Hydrogenase from Ralstonia eutropha H16 by Surface-Enhanced IR Absorption Spectroscopy †
Article first published online: 9 DEC 2008
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 48, Issue 3, pages 611–613, January 5, 2009
How to Cite
Wisitruangsakul, N., Lenz, O., Ludwig, M., Friedrich, B., Lendzian, F., Hildebrandt, P. and Zebger, I. (2009), Monitoring Catalysis of the Membrane-Bound Hydrogenase from Ralstonia eutropha H16 by Surface-Enhanced IR Absorption Spectroscopy . Angew. Chem. Int. Ed., 48: 611–613. doi: 10.1002/anie.200802633
- Issue published online: 29 DEC 2008
- Article first published online: 9 DEC 2008
- Manuscript Received: 4 JUN 2008
- hydrogen splitting;
- SEIRA spectroscopy
Immobilized biocatalyst: The oxygen-tolerant, membrane-bound hydrogenase of Ralstonia eutropha H16 is immobilized by a His-tag (see picture; green) onto a gold surface (yellow) modified with nickel–nitrilotriacetic acid (red/black). Catalytic activity towards hydrogen is investigated by surface-enhanced infrared absorption (SEIRA) spectroscopy. Switching redox states and related structural changes of the Ni–Fe active site are followed by the CO and CN− ligand stretching modes.