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Keywords:

  • biocatalysis;
  • biofuels;
  • hydrogen splitting;
  • hydrogenase;
  • SEIRA spectroscopy
Thumbnail image of graphical abstract

Immobilized biocatalyst: The oxygen-tolerant, membrane-bound hydrogenase of Ralstonia eutropha H16 is immobilized by a His-tag (see picture; green) onto a gold surface (yellow) modified with nickel–nitrilotriacetic acid (red/black). Catalytic activity towards hydrogen is investigated by surface-enhanced infrared absorption (SEIRA) spectroscopy. Switching redox states and related structural changes of the Ni–Fe active site are followed by the CO and CN ligand stretching modes.