Communication

Monitoring Catalysis of the Membrane-Bound Hydrogenase from Ralstonia eutropha H16 by Surface-Enhanced IR Absorption Spectroscopy †

  1. Nattawadee Wisitruangsakul1,
  2. Oliver Lenz Dr.2,
  3. Marcus Ludwig Dr.2,
  4. Bärbel Friedrich Prof.2,
  5. Friedhelm Lendzian Dr.1,
  6. Peter Hildebrandt Prof.1,
  7. Ingo Zebger Dr.1

Article first published online: 9 DEC 2008

DOI: 10.1002/anie.200802633

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 48, Issue 3, pages 611–613, January 5, 2009

Additional Information

How to Cite

Wisitruangsakul, N., Lenz, O., Ludwig, M., Friedrich, B., Lendzian, F., Hildebrandt, P. and Zebger, I. (2009), Monitoring Catalysis of the Membrane-Bound Hydrogenase from Ralstonia eutropha H16 by Surface-Enhanced IR Absorption Spectroscopy . Angew. Chem. Int. Ed., 48: 611–613. doi: 10.1002/anie.200802633

Author Information

  1. 1

    Institut für Chemie, Sekr. PC14, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin (Germany), Fax: (+49) 30-31421122

  2. 2

    Institut f. Biologie/Mikrobiologie, Humboldt Universität zu Berlin, Chausseestrasse 117, 10115 Berlin (Germany)

  1. †

    The work was supported by the DFG (SFB 498, Cluster of Excellence “Unicat”).

Publication History

  1. Issue published online: 29 DEC 2008
  2. Article first published online: 9 DEC 2008
  3. Manuscript Received: 4 JUN 2008

Funded by

  • DFG

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Keywords:

  • biocatalysis;
  • biofuels;
  • hydrogen splitting;
  • hydrogenase;
  • SEIRA spectroscopy
Thumbnail image of graphical abstract

Immobilized biocatalyst: The oxygen-tolerant, membrane-bound hydrogenase of Ralstonia eutropha H16 is immobilized by a His-tag (see picture; green) onto a gold surface (yellow) modified with nickel–nitrilotriacetic acid (red/black). Catalytic activity towards hydrogen is investigated by surface-enhanced infrared absorption (SEIRA) spectroscopy. Switching redox states and related structural changes of the Ni–Fe active site are followed by the CO and CN− ligand stretching modes.

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