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Monitoring Catalysis of the Membrane-Bound Hydrogenase from Ralstonia eutropha H16 by Surface-Enhanced IR Absorption Spectroscopy


  • The work was supported by the DFG (SFB 498, Cluster of Excellence “Unicat”).


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Immobilized biocatalyst: The oxygen-tolerant, membrane-bound hydrogenase of Ralstonia eutropha H16 is immobilized by a His-tag (see picture; green) onto a gold surface (yellow) modified with nickel–nitrilotriacetic acid (red/black). Catalytic activity towards hydrogen is investigated by surface-enhanced infrared absorption (SEIRA) spectroscopy. Switching redox states and related structural changes of the Ni–Fe active site are followed by the CO and CN ligand stretching modes.