The Absolute Configuration of Rhizopodin and Its Inhibition of Actin Polymerization by Dimerization


  • This work was supported by the Deutsche Forschungsgemeinschaft as part of the Priority Program 1150 (SCHU 1560/1-1 to 1-3). Beam time and support by X12 beamline staff (EMBL Hamburg outstation, DESY) is gratefully acknowledged. Coordinates of the actin/rhizopodin complex have been deposited in the Protein Data Bank under access number 2VYP.


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Three's company: Rhizopodin is a cytostatic macrolide and a potent actin depolymerizer produced by the myxobacterium Myxococcus stipitatus. A crystal structure analysis of the rhizopodin/actin complex reveals that rhizopodin is a C2-symmetric bislactone (see formula). The ternary complex supports the mode of rhizopodin-induced actin dimerization and reveals the absolute configuration and biologically active conformation of this macrolide.