We acknowledge support from the Deutsche Forschungsgemeinschaft and Schering AG.
Low-Noise Stemless PNA Beacons for Sensitive DNA and RNA Detection†
Article first published online: 23 OCT 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 47, Issue 49, pages 9555–9559, November 24, 2008
How to Cite
Socher, E., Bethge, L., Knoll, A., Jungnick, N., Herrmann, A. and Seitz, O. (2008), Low-Noise Stemless PNA Beacons for Sensitive DNA and RNA Detection. Angew. Chem. Int. Ed., 47: 9555–9559. doi: 10.1002/anie.200803549
- Issue published online: 20 NOV 2008
- Article first published online: 23 OCT 2008
- Manuscript Received: 21 JUL 2008
- Deutsche Forschungsgemeinschaft
- Schering AG
- molecular beacons;
- single-nucleotide polymorphism
A change of the energy transfer mechanism (from contact quenching to FRET) increases the responsiveness of fluorescent hybridization probes. The communication between a responsive fluorescent base surrogate (see picture, green) and a near-infrared dye (red) in peptide nucleic acid provides probes that are extremely dark in the single strand. Hybridization furnishes strong fluorescence increase with a 200 nm shift in emission maximum.