This research was supported in part by the MEXT (Japan) through Grants-in-Aid for Scientific Research and the Academic Frontier Project (2004–2009), and by the Hirao Taro Foundation of the Konan University Association for Academic Research.
Regulation of Telomerase Activity by the Thermodynamic Stability of a DNA⋅RNA Hybrid†
Article first published online: 10 OCT 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 47, Issue 47, pages 9034–9038, November 10, 2008
How to Cite
Yu, H.-Q., Zhang, D.-H., Gu, X.-B., Miyoshi, D. and Sugimoto, N. (2008), Regulation of Telomerase Activity by the Thermodynamic Stability of a DNA⋅RNA Hybrid. Angew. Chem. Int. Ed., 47: 9034–9038. doi: 10.1002/anie.200803577
- Issue published online: 5 NOV 2008
- Article first published online: 10 OCT 2008
- Manuscript Received: 23 JUL 2008
- MEXT (Japan)
- Hirao Taro Foundation of the Konan University Association for Academic Research
- DNA-RNA hybrids;
- molecular crowding;
Slide or stick: The thermodynamic stability of a hybrid composed of substrate DNA and template RNA derived from telomerase was investigated under dilute and molecular-crowding conditions. Destabilization of the hybrid by cosolutes of low molecular weight (MW) leads to an easier sliding of telomerase along the telomeric DNA (see picture). In contrast, telomerase tends to stick to the telomeric DNA when the hybrid is stabilized by high-MW cosolutes.