Regulation of Telomerase Activity by the Thermodynamic Stability of a DNA⋅RNA Hybrid

Authors

  • Hai-Qing Yu Dr.,

    1. Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501 (Japan), Fax: (+81) 078-435-2766
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  • Dong-Hao Zhang Dr.,

    1. Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501 (Japan), Fax: (+81) 078-435-2766
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  • Xiao-Bo Gu Dr.,

    1. Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501 (Japan), Fax: (+81) 078-435-2766
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  • Daisuke Miyoshi Dr.,

    1. Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501 (Japan), Fax: (+81) 078-435-2766
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  • Naoki Sugimoto Prof.

    1. Department of Chemistry, Faculty of Science and Engineering, and Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501 (Japan), Fax: (+81) 78-435-2539
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  • This research was supported in part by the MEXT (Japan) through Grants-in-Aid for Scientific Research and the Academic Frontier Project (2004–2009), and by the Hirao Taro Foundation of the Konan University Association for Academic Research.

Abstract

original image

Slide or stick: The thermodynamic stability of a hybrid composed of substrate DNA and template RNA derived from telomerase was investigated under dilute and molecular-crowding conditions. Destabilization of the hybrid by cosolutes of low molecular weight (MW) leads to an easier sliding of telomerase along the telomeric DNA (see picture). In contrast, telomerase tends to stick to the telomeric DNA when the hybrid is stabilized by high-MW cosolutes.

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