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Proteomic Analysis of Plasma Membrane Vesicles

Authors


  • This work is supported by the Royal Swedish Academy of Sciences, the Swedish Research Council (VR), the Swedish Foundation for Strategic Research (SSF) through the NANO-X program, and the Göran Gustafsson Foundation.

Abstract

A simple and scalable method is presented for harvesting, purification, and on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flowcell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by LC-MS/MS. Over 93 % of the detected proteins are plasma-membrane-derived.

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