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Self-Assembly of One- and Two-Dimensional Hemoprotein Systems by Polymerization through Heme–Heme Pocket Interactions

Authors

  • Hiroaki Kitagishi Dr.,

    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871 (Japan), Fax: (+81) 6-6879-7930
    2. Present address: Department of Molecular Chemistry and Biochemistry, Doshisha University, Kyotanabe, Kyoto 610-0321 (Japan)
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  • Yasuaki Kakikura,

    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871 (Japan), Fax: (+81) 6-6879-7930
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  • Hiroyasu Yamaguchi Dr.,

    1. Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka 560-0043 (Japan)
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  • Koji Oohora,

    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871 (Japan), Fax: (+81) 6-6879-7930
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  • Akira Harada Prof. Dr.,

    1. Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka 560-0043 (Japan)
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  • Takashi Hayashi Prof. Dr.

    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871 (Japan), Fax: (+81) 6-6879-7930
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  • This work was supported by Grants-in-Aid for Scientific Research from MEXT. T.H. acknowledges a research grant from the Asahi Glass Foundation. H.K. was financially supported as an Inoue Fellow of the Inoue Foundation for Science.

Abstract

Supramolecular protein polymers: When a heme moiety was introduced to the surface of an apo-cytochrome b562(H63C) mutant, supramolecular polymers formed through noncovalent heme–heme pocket interactions. The incorporation of a heme triad as a pivot molecule in the protein polymer further led to a two-dimensional protein network structure, which was visualized by tapping-mode atomic force microscopy (see picture).

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