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Shuttle-Based Fluorogenic Silver-Cluster Biolabels

Authors

  • Junhua Yu Dr.,

    1. School of Chemistry and Biochemistry and Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332-0400 (USA), Fax: (+1) 404-894-7452
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  • Sungmoon Choi,

    1. School of Chemistry and Biochemistry and Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332-0400 (USA), Fax: (+1) 404-894-7452
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  • Robert M. Dickson Prof.

    1. School of Chemistry and Biochemistry and Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332-0400 (USA), Fax: (+1) 404-894-7452
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  • The authors gratefully acknowledge financial support from Invitrogen Corp. and NIH R01-GM068732. We also thank Prof. D. F. Doyle and Prof. C. J. Fahrni (Georgia Tech) for use of their cell culture facility, and Prof. Jens Karlsson (Drexel University) for a gift of BPAECs.

Abstract

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Clustering together: A shuttle-based fluorogenic cluster transfer, in which silver clusters (SCs) are directly transferred from a low-molecular-weight poly(acrylic acid) (PA) shuttle to a single-stranded DNA (ssDNA) tag on the protein of interest (see picture), proceeds with excellent specificity. Upon transfer, the cluster fluorescence increases more than ten times, which provides bright, photostable labeling.

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