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Keywords:

  • droplets;
  • enzymatic amplification;
  • high-throughput screening;
  • microfluidics;
  • proteins

Abstract

Thumbnail image of graphical abstract

Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.