Communication

Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets†

  1. Haakan N. Joensson1,
  2. Michael L. Samuels Dr.2,
  3. Eric R. Brouzes Dr.2,
  4. Martina Medkova Dr.2,
  5. Mathias Uhlén Prof.3,
  6. Darren R. Link Dr.2,
  7. Helene Andersson-Svahn Prof.1

Article first published online: 23 FEB 2009

DOI: 10.1002/anie.200804326

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 48, Issue 14, pages 2518–2521, March 23, 2009

Additional Information

How to Cite

Joensson, Haakan N., Samuels, Michael L., Brouzes, Eric R., Medkova, M., Uhlén, M., Link, Darren R. and Andersson-Svahn, H. (2009), Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets. Angew. Chem. Int. Ed., 48: 2518–2521. doi: 10.1002/anie.200804326

Author Information

  1. 1

    Department of Nano Biotechnology, Royal Institute of Technology, AlbaNova University Center, 10691 Stockholm (Sweden), Fax: (+46) 8-5537-8323

  2. 2

    RainDance Technologies, 44 Hartwell Ave, Lexington MA 02421 (USA)

  3. 3

    Department of Proteomics, Royal Institute of Technology, AlbaNova University Center, 10691 Stockholm (Sweden)

  1. †

    This work was supported in part by NIH grant R43HG003925-01, The Knut and Alice Wallenberg Foundation, The Royal Swedish Academy of Sciences and Swedish Research Council.

Publication History

  1. Issue published online: 17 MAR 2009
  2. Article first published online: 23 FEB 2009
  3. Manuscript Revised: 22 DEC 2008
  4. Manuscript Received: 2 SEP 2008

Funded by

  • NIH. Grant Number: R43HG003925-01
  • The Knut and Alice Wallenberg Foundation
  • The Royal Swedish Academy of Sciences and Swedish Research Council

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Get PDF (485K)

Keywords:

  • droplets;
  • enzymatic amplification;
  • high-throughput screening;
  • microfluidics;
  • proteins

Abstract

Thumbnail image of graphical abstract

Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.

View Full Article with Supporting Information (HTML) Get PDF (485K)