P.K. thanks the Humboldt Foundation for a Humboldt fellowship (3.3-UNG/1126507). Financial support from GVOP-3.2.1.-2004-04-0005/3.0, T 049814 (OTKA) and H07-B-74291 (NKTH-OTKA) are greatly acknowledged. The authors wish to thank Dr. K. Hegyi and Dr. Z. Novák for helpful discussions.
Dual Labeling of Biomolecules by Using Click Chemistry: A Sequential Approach†
Article first published online: 9 DEC 2008
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 48, Issue 2, pages 344–347, January 2, 2009
How to Cite
Kele, P., Mezö, G., Achatz, D. and Wolfbeis, Otto S. (2009), Dual Labeling of Biomolecules by Using Click Chemistry: A Sequential Approach. Angew. Chem. Int. Ed., 48: 344–347. doi: 10.1002/anie.200804514
- Issue published online: 22 DEC 2008
- Article first published online: 9 DEC 2008
- Manuscript Received: 13 SEP 2008
- Humboldt Foundation. Grant Numbers: 3.3-UNG/1126507, GVOP-3.2.1.-2004-04-0005/3.0, T 049814
- OTKA. Grant Number: H07-B-74291
- click chemistry;
Click by click: Dual labeling of model compounds was carried out by using copper-free and copper-mediated click chemistry in a sequential manner. This method was used to introduce two labels onto biological targets or nanoparticles, thus quickly converting them into fluorescence resonance energy transfer systems.