Triggered Enzymatic Degradation of DNA within Selectively Permeable Polymer Capsule Microreactors

Authors

  • Andrew D. Price Dr.,

    1. Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010 (Australia), Fax: (+61) 3-8344-4153
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  • Alexander N. Zelikin Dr.,

    1. Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010 (Australia), Fax: (+61) 3-8344-4153
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  • Yajun Wang Dr.,

    1. Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010 (Australia), Fax: (+61) 3-8344-4153
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  • Frank Caruso Prof.

    1. Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010 (Australia), Fax: (+61) 3-8344-4153
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  • This research was supported by the Australian Research Council. We thank Dr. B. Städler for her assistance with TEM.

Abstract

original image

Thinking in compartments is back! The degradation of a fluorescently labeled nucleic acid substrate within a polymer capsule by coencapsulated DNase I was initiated by activation of the enzyme with a chemical trigger. The degraded DNA diffused from the capsule (see picture). The semipermeable capsule microreactors enable external reaction control, high-throughput tracking of reaction progression, and continuous modification of the DNA cargo.

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