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Hydrocyanines: A Class of Fluorescent Sensors That Can Image Reactive Oxygen Species in Cell Culture, Tissue, and In Vivo

Authors

  • Kousik Kundu,

    1. The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332 (USA), Fax: (+1) 404-894-4243
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  • Sarah F. Knight,

    1. Cardiology Division, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322 (USA)
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  • Nick Willett,

    1. Cardiology Division, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322 (USA)
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  • Sungmun Lee,

    1. The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332 (USA), Fax: (+1) 404-894-4243
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  • W. Robert Taylor Prof.,

    1. The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332 (USA), Fax: (+1) 404-894-4243
    2. Cardiology Division, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322 (USA)
    3. Cardiology Division, Atlanta VA Medical Center, Decatur, GA 30032 (USA)
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  • Niren Murthy Prof.

    1. The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332 (USA), Fax: (+1) 404-894-4243
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  • This work was supported by the Georgia Tech/Emory Center for the Engineering of Living Tissues (funded by NSF-EEC-9731643) (N.M.), NSF-BES-0546962 Career Award (N.M.), NIH UO1 HL80711-01 (N.M.), NIH R21 EB006418 (N.M.), and a J&J/GT Health Care Innovation Seed Grant Proposal (N.M.).

Abstract

original image

Accurate and tunable: The title compounds can detect reactive oxygen species (ROS) in cell culture, tissue explants, and for the first time in vivo. The hydrocyanines are synthesized by reduction of the cyanine dyes with NaBH4. They can accurately detect nanomolar levels of ROS, have excellent stability against autoxidation, and have tunable emission wavelengths in the range 560–830 nm.

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