This work was supported the National Institutes of Health (GM35208). D.M. thanks The Human Frontier Science Program Organization for a cross-disciplinary postdoctoral fellowship. We thank NanoDrop for the use of a NanoDrop 3300 Fluorospectrometer.
1521-3773/asset/2002_left.gif?v=1&s=ac6b0d94a94d7ce7a210002b8096b42feffc0bcf)
1521-3773/asset/2002_right.gif?v=1&s=451042aa3415ae3ad0729984d26dee1866aca82e)
Communication
Protein Recognition by an Ensemble of Fluorescent DNA G-Quadruplexes†
Article first published online: 28 JAN 2009
DOI: 10.1002/anie.200804887
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
Angewandte Chemie International Edition
Volume 48, Issue 10, pages 1771–1774, February 23, 2009
Additional Information
How to Cite
Margulies, D. and Hamilton, Andrew D. (2009), Protein Recognition by an Ensemble of Fluorescent DNA G-Quadruplexes. Angew. Chem. Int. Ed., 48: 1771–1774. doi: 10.1002/anie.200804887
- †
Publication History
- Issue published online: 17 FEB 2009
- Article first published online: 28 JAN 2009
- Manuscript Received: 6 OCT 2008
Funded by
- National Institutes of Health. Grant Number: GM35208
- The Human Frontier Science Program Organization
- NanoDrop
Keywords:
- DNA;
- fluorescence;
- proteins;
- self-assembly;
- supramolecular chemistry
Abstract
Sniffing out proteins: Fluorescent DNA G-quadruplexes have been used for building versatile signaling receptors for proteins in a single solution. Introducing a protein sample to the ensemble results in a unique emission signature for unambiguous identification (see scheme, R=fluorophore). The self-assembled, pattern-based protein detection systems are easily fabricated, have the potential for high-throughput operations, and have the ability to handle small protein samples.