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Protein Recognition by an Ensemble of Fluorescent DNA G-Quadruplexes


  • This work was supported the National Institutes of Health (GM35208). D.M. thanks The Human Frontier Science Program Organization for a cross-disciplinary postdoctoral fellowship. We thank NanoDrop for the use of a NanoDrop 3300 Fluorospectrometer.


Sniffing out proteins: Fluorescent DNA G-quadruplexes have been used for building versatile signaling receptors for proteins in a single solution. Introducing a protein sample to the ensemble results in a unique emission signature for unambiguous identification (see scheme, R=fluorophore). The self-assembled, pattern-based protein detection systems are easily fabricated, have the potential for high-throughput operations, and have the ability to handle small protein samples.

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