Communication

Plug-Based Microfluidics with Defined Surface Chemistry to Miniaturize and Control Aggregation of Amyloidogenic Peptides†

  1. Matthias Meier Dr.1,
  2. Julia Kennedy-Darling1,
  3. Se Hoon Choi2,
  4. Eric M. Norstrom Dr.2,
  5. Sangram S. Sisodia Prof. Dr.2,
  6. Rustem F. Ismagilov Prof. Dr.1

Article first published online: 16 JAN 2009

DOI: 10.1002/anie.200805225

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 48, Issue 8, pages 1487–1489, February 9, 2009

Additional Information

How to Cite

Meier, M., Kennedy-Darling, J., Choi, S., Norstrom, Eric M., Sisodia, Sangram S. and Ismagilov, Rustem F. (2009), Plug-Based Microfluidics with Defined Surface Chemistry to Miniaturize and Control Aggregation of Amyloidogenic Peptides. Angew. Chem. Int. Ed., 48: 1487–1489. doi: 10.1002/anie.200805225

Author Information

  1. 1

    Department of Chemistry, University of Chicago, 929 East 57th Street, Chicago, IL 60637 (USA), Fax: (+1) 773-834-3544 http://ismagilovlab.uchicago.edu

  2. 2

    The Center for Molecular Neurobiology, University of Chicago, 947 E 58th Street, Chicago, IL 60637 (USA)

  1. †

    This research was supported by the National Institutes of Health NIBIB EB000557 and the NIH Director's Pioneer Award DP1 OD003584 to R.F.I. and by Cure Alzheimer's Fund and NIH AG027854 to S.S.S. R.F.I. is a Camille Dreyfus Teacher-Scholar. M.M. was supported by the Alexander von Humboldt Society and J.K.-D. by the NIH Roadmap training program T90 DK070076. We thank Dr. A. M. Seddon and Dr. E. Chan for preliminary experiments and E. W. Haney for editing this manuscript.

Publication History

  1. Issue published online: 30 JAN 2009
  2. Article first published online: 16 JAN 2009
  3. Manuscript Received: 25 OCT 2008

Funded by

  • National Institutes of Health. Grant Numbers: NIBIB EB000557, DP1 OD003584
  • Cure Alzheimer's Fund. Grant Number: NIH AG027854
  • Alexander von Humboldt Society. Grant Number: T90 DK070076

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Keywords:

  • aggregation;
  • Alzheimer's disease;
  • interfaces;
  • microfluidics;
  • proteins

Abstract

Thumbnail image of graphical abstract

Small with control: For miniaturization of protein aggregation experiments the interfacial chemistry must be controlled to avoid protein aggregation caused by interfacial adsorption. Plug-based microfluidics with defined surface chemistry (see schematic picture) can then be used to perform hundreds of aggregation experiments with volume-limited samples, such as cerebrospinal fluid from mice.

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