This work and use of the Advanced Photon Source, was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences under contract numbers DE-FG02-07ER15865 (C.H.) and DE-AC02-06CH11357 (M.P.J. and APS use), and a UChicago–Argonne Strategic Collaborative Initiative grant (C.H. and M.P.J.). We thank P. T. Chivers for the nikR encoding plasmid and are grateful for the use of XOR/BESSRC-CAT and the Actinide Facility. NikR is a Ni2+-dependent transcriptional repressor protein.
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Communication
Engineering A Uranyl-Specific Binding Protein from NikR†
Article first published online: 6 FEB 2009
DOI: 10.1002/anie.200805262
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Wegner, S., Boyaci, H., Chen, H., Jensen, M. and He, C. (2009), Engineering A Uranyl-Specific Binding Protein from NikR. Angewandte Chemie International Edition, 48: 2339–2341. doi: 10.1002/anie.200805262
- †
Publication History
- Issue published online: 10 MAR 2009
- Article first published online: 6 FEB 2009
- Manuscript Received: 28 OCT 2008
Funded by
- US Department of Energy, Office of Science, Office of Basic Energy Sciences. Grant Numbers: DE-FG02-07ER15865, DE-AC02-06CH11357
Keywords:
- bioinorganic chemistry;
- metalloproteins;
- nickel;
- protein engineering;
- uranium
Graphical Abstract
A new pick-up line: The first uranyl-selective DNA-binding protein is designed using the E. coli nickel(II)-responsive protein NikR as the template. The resulting NikR′ protein binds uranyl (see picture) with a dissociation constant Kd=53 nM and selectively binds to DNA in the presence of uranyl.
Abstract
A new pick-up line: The first uranyl-selective DNA-binding protein is designed using the E. coli nickel(II)-responsive protein NikR as the template. The resulting NikR′ protein binds uranyl (see picture) with a dissociation constant Kd=53 nM and selectively binds to DNA in the presence of uranyl.