We thank the Deutsche Forschungsgemeinschaft (DFG), the Volkswagen Foundation, the Fonds der Chemischen Industrie, and the TU Dortmund for financial support.
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Bioorthogonal Ligation in the Spotlight†
Article first published online: 15 JAN 2009
DOI: 10.1002/anie.200805454
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
Angewandte Chemie International Edition
Volume 48, Issue 10, pages 1729–1731, February 23, 2009
Additional Information
How to Cite
Kurpiers, T. and Mootz, Henning D. (2009), Bioorthogonal Ligation in the Spotlight. Angew. Chem. Int. Ed., 48: 1729–1731. doi: 10.1002/anie.200805454
- †
Publication History
- Issue published online: 17 FEB 2009
- Article first published online: 15 JAN 2009
Funded by
- Deutsche Forschungsgemeinschaft (DFG)
- Volkswagen Foundation
- Fonds der Chemischen Industrie
- Abstract
- Article
- References
- Cited By
Keywords:
- alkenes;
- cycloaddition;
- fluorescent probes;
- photochemistry;
- protein modifications
Abstract
The light-induced formation of a covalent bond between an alkene group and a tetrazole moiety has been used for the selective labeling of proteins in vitro and in live cells. This bioorthogonal ligation initiated by brief irradiation with UV light leads to a fluorescent pyrazoline adduct (see scheme).