We thank Heike Stichnoth for providing cells and Dr. Adrian Neal for providing TBTA and 3-azido-7-(diethylamino)coumarin.
1521-3773/asset/2002_left.gif?v=1&s=ac6b0d94a94d7ce7a210002b8096b42feffc0bcf)
1521-3773/asset/2002_right.gif?v=1&s=451042aa3415ae3ad0729984d26dee1866aca82e)
Communication
Selective Fluorescence Labeling of Lipids in Living Cells†
Article first published online: 14 JAN 2009
DOI: 10.1002/anie.200805507
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Neef, Anne B. and Schultz, C. (2009), Selective Fluorescence Labeling of Lipids in Living Cells. Angew. Chem. Int. Ed., 48: 1498–1500. doi: 10.1002/anie.200805507
- †
Publication History
- Issue published online: 30 JAN 2009
- Article first published online: 14 JAN 2009
- Manuscript Received: 11 NOV 2008
Keywords:
- alkynes;
- click chemistry;
- fluorescent probes;
- phospholipids
Abstract
Click chemistry in vivo: Three phosphatidic acid derivatives with alkyne groups in their fatty acid chains were synthesized and incorporated into mammalian cell membranes. Copper(I)-catalyzed and strain-promoted azide–alkyne cycloaddition reactions were used for their visualization (see schematic representation and fluorescence microscopic image).