Synthesis and Microcontact Printing of Dual End-Functionalized Mucin-like Glycopolymers for Microarray Applications

Authors

  • Kamil Godula,

    1. Department of Chemistry, University of California and The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA)
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  • David Rabuka,

    1. Department of Chemistry, University of California and The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA)
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  • Ki Tae Nam,

    1. Department of Chemistry, University of California and The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA)
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  • Carolyn R. Bertozzi Prof.

    1. Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California and The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA), Fax: (+1) 510-643-2628
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Errata

This article is corrected by:

  1. Errata: Corrigendum: Synthesis and Microcontact Printing of Dual End-Functionalized Mucin-like Glycopolymers for Microarray Applications Volume 51, Issue 32, 7881, Article first published online: 1 August 2012

  • This work was supported by the Director, Office of Energy Research, Office of Basic Energy Sciences, Division of Materials Sciences, of the U.S. Department of Energy under Contract No. DE-AC03-76SF00098, within the Interfacing Nanostructures Initiative and NIH (K99M080585-01). Portions of this work were performed at the Molecular Foundry, Lawrence Berkeley National Laboratory, which is supported by the Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. We thank Dr. Ramesh Jasti for stimulating discussions, Dr. Marian Snauko for technical support, and Prof. Pil J. Yoo of SKKU Advanced Institute of Nanotechnology for graciously providing PDMS stamps.

Abstract

Click to view: Glycopolymers can be used to display glycans on microarrays in native-like architectures. The structurally uniform alkyne-terminated mucin mimetic glycopolymers (see picture; TR=fluorophore) were printed on azide-functionalized chips by microcontact printing in the presence of a copper catalyst. The surface-bound glycopolymers bind lectins in a ligand-specific manner.

original image

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