Communication

Fast Biosynthesis of GFP Molecules: A Single-Molecule Fluorescence Study†

  1. Alexandros Katranidis1,2,
  2. Diaa Atta1,
  3. Ramona Schlesinger1,
  4. Knud H. Nierhaus3,
  5. Theodora Choli-Papadopoulou2,
  6. Ingo Gregor4,6,
  7. Michael Gerrits5,
  8. Georg Büldt1,
  9. Jörg Fitter1

Article first published online: 28 JAN 2009

DOI: 10.1002/anie.200806070

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 48, Issue 10, pages 1758–1761, February 23, 2009

Additional Information

How to Cite

Katranidis, A., Atta, D., Schlesinger, R., Nierhaus, Knud H., Choli-Papadopoulou, T., Gregor, I., Gerrits, M., Büldt, G. and Fitter, J. (2009), Fast Biosynthesis of GFP Molecules: A Single-Molecule Fluorescence Study. Angew. Chem. Int. Ed., 48: 1758–1761. doi: 10.1002/anie.200806070

Author Information

  1. 1

    Forschungszentrum Jülich, ISB-2: Molecular Biophysics, 52425 Jülich (Germany), Fax: (+49) 2461-612-020

  2. 2

    Laboratory of Biochemistry, Aristotle University of Thessaloniki (Greece)

  3. 3

    Max-Planck-Institut für molekulare Genetik, Berlin (Germany)

  4. 4

    Forschungszentrum Jülich, ISB-1: Cellular Biophysics, Jülich (Germany)

  5. 5

    RiNA GmbH, Takustrasse 3, 14195 Berlin (Germany)

  6. 6

    Present address: Forschungszentrum caesar, Abt. Molekulare Neurosensorik, Bonn (Germany)

  1. †

    J. Groll (SusTech Darmstadt (Germany)) is acknowledged for providing us with a protocol to prepare cover slides for the use of single surface-tethered biomolecules. We thank J. Enderlein and B. Kaupp for supporting us with a two-color fluorescence wide-field microscope setup. We thank W. Stiege from RiNA GmbH Berlin for generous support with cell-free expression. GFP=green fluorescent protein.

Publication History

  1. Issue published online: 17 FEB 2009
  2. Article first published online: 28 JAN 2009
  3. Manuscript Received: 12 DEC 2008

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Keywords:

  • fluorescence microscopy;
  • green fluorescent proteins;
  • protein folding;
  • ribosomes;
  • single-molecule studies

Abstract

Thumbnail image of graphical abstract

It's not easy being green: Real-time visualization of labeled ribosomes and de novo synthesized green fluorescent protein molecules using single-molecule-sensitive fluorescence microscopy demonstrates that the mutant GFPem is produced with a characteristic time of five minutes. Fluorescence of the fastest GFP molecules appears within one minute (see picture).

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