This work was supported in part by the NIH grants: R21 NS055845 (G.B.) and Program Project GM 48677 (B.M.O., G.B., D.Y.). We thank Drs. Robert Schackmann and Scott Endicott from the DNA/Peptide Synthesis Core for their help with chemical synthesis.
Integrated Oxidative Folding of Cysteine/Selenocysteine Containing Peptides: Improving Chemical Synthesis of Conotoxins†
Article first published online: 10 FEB 2009
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 48, Issue 12, pages 2221–2224, March 9, 2009
How to Cite
Walewska, A., Zhang, M.-M., Skalicky, Jack J., Yoshikami, D., Olivera, Baldomero M. and Bulaj, G. (2009), Integrated Oxidative Folding of Cysteine/Selenocysteine Containing Peptides: Improving Chemical Synthesis of Conotoxins. Angew. Chem. Int. Ed., 48: 2221–2224. doi: 10.1002/anie.200806085
- Issue published online: 3 MAR 2009
- Article first published online: 10 FEB 2009
- Manuscript Received: 13 DEC 2008
- NIH. Grant Number: R21 NS055845
- NMR spectroscopy;
- oxidative folding;
Building bridges: The use of diselenide and selectively (15N/13C)-labeled disulfide bridges is combined to give improvements in oxidative folding and disulfide mapping. Conotoxin analogues, each with a pair of selenocysteines (Sec) and labeled cysteines (see scheme, red), exhibited significantly improved folding and the labeled cysteines allow correctly folded species to be rapidly identified by NMR spectroscopy.