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Keywords:

  • conotoxin;
  • cysteine;
  • NMR spectroscopy;
  • oxidative folding;
  • selenium

Abstract

Thumbnail image of graphical abstract

Building bridges: The use of diselenide and selectively (15N/13C)-labeled disulfide bridges is combined to give improvements in oxidative folding and disulfide mapping. Conotoxin analogues, each with a pair of selenocysteines (Sec) and labeled cysteines (see scheme, red), exhibited significantly improved folding and the labeled cysteines allow correctly folded species to be rapidly identified by NMR spectroscopy.