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Mass Spectrometry Assisted Lithography for the Patterning of Cell Adhesion Ligands on Self-Assembled Monolayers

Authors

  • Young-Kwan Kim,

    1. Department of Chemistry and School of Molecular Science (BK21), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701 (Korea), Fax: (+82) 42-350-2810
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  • Soo-Ryoon Ryoo,

    1. Department of Chemistry and School of Molecular Science (BK21), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701 (Korea), Fax: (+82) 42-350-2810
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  • Sul-Jin Kwack,

    1. Department of Chemistry and School of Molecular Science (BK21), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701 (Korea), Fax: (+82) 42-350-2810
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  • Dal-Hee Min Prof. Dr.

    1. Department of Chemistry and School of Molecular Science (BK21), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701 (Korea), Fax: (+82) 42-350-2810
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  • This work was supported by the Korea Science & Engineering Foundation (KOSEF) through a general research grant (no. R01-2008-000-20301-0) and by the nano R&D program (no. 2008-04457) funded by the Korean government. We would like to thank Prof. Dr. Hee Cheul Choi at POSTECH for gold substrates and Prof. Dr. Ryong Ryoo, Prof. Dr. Juhyoun Kwack, and Prof. Dr. Yoonkey Nam at KAIST for their help with obtaining cell images. We thank Prof. Dr. Woon-Seok Yeo at Konkuk University for helpful discussions.

Abstract

Pattern of events: A simple and flexible method has been developed for patterning cell adhesion ligands. Locally erasing self-assembled monolayers with tri(ethyleneglycol) groups on a gold substrate by using a MALDI-TOF MS nitrogen laser and filling the exposed gold surface with an alkanethiol presenting carboxylic acid groups enables subsequent immobilization of maleimide and a cell adhesion peptide, which can then recognize cells (see scheme).

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