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Keywords:

  • amyloids;
  • inclusion bodies;
  • NMR spectroscopy;
  • proteins;
  • structure elucidation

Abstract

Thumbnail image of graphical abstract

Protein deposition frequently occurs as inclusion bodies (IBs) during heterologous protein expression in E. coli. The structure of these E. coli IBs of the prion-forming domain from the fungal prion HET-s is the same as that previously determined for fibrils assembled in vitro, and show prion infectivity. These results demonstrate that the IBs of HET-s(218–289) are amyloids.