Communication

A Xenon-129 Biosensor for Monitoring MHC–Peptide Interactions†

  1. Andreas Schlundt1,
  2. Wolfgang Kilian Dr.2,
  3. Michael Beyermann Dr.3,
  4. Jana Sticht Dr.1,
  5. Sebastian Günther4,5,
  6. Sabine Höpner4,5,
  7. Kirsten Falk Dr.4,5,
  8. Olaf Roetzschke Dr.4,5,
  9. Lorenz Mitschang Dr.2,
  10. Christian Freund Dr.1

Article first published online: 30 APR 2009

DOI: 10.1002/anie.200806149

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 48, Issue 23, pages 4142–4145, May 25, 2009

Additional Information

How to Cite

Schlundt, A., Kilian, W., Beyermann, M., Sticht, J., Günther, S., Höpner, S., Falk, K., Roetzschke, O., Mitschang, L. and Freund, C. (2009), A Xenon-129 Biosensor for Monitoring MHC–Peptide Interactions. Angewandte Chemie International Edition, 48: 4142–4145. doi: 10.1002/anie.200806149

Author Information

  1. 1

    Protein Engineering Group, Leibniz-Institut für Molekulare Pharmakologie und FU Berlin, Robert-Roessle-Strasse 10, 13125 Berlin (Germany), Fax: (+49) 30-94793-189

  2. 2

    Physikalisch-Technische Bundesanstalt, Berlin (Germany)

  3. 3

    Peptide Chemistry Group, Leibniz-Institut für Molekulare Pharmakologie und FU Berlin

  4. 4

    Max-Delbrück-Centrum für Molekulare Medizin, Berlin (Germany)

  5. 5

    The Singapore Immunology Network, Singapore 138648 (Singapore)

  1. †

    We thank Peter Schmieder for helpful discussions, Bernhard Schmikale for synthesizing cage-coupled HA peptide, and Werner Hoffmann and Karl-Heinz Rhein for probe coil design. This work was supported by grant 01GU0514 of the BMBF to C.F., K.F., and O.R. MHC=major histocompatibility complex.

Publication History

  1. Issue published online: 19 MAY 2009
  2. Article first published online: 30 APR 2009
  3. Manuscript Revised: 12 MAR 2009
  4. Manuscript Received: 17 DEC 2008

Funded by

  • BMBF

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Keywords:

  • antigens;
  • biosensors;
  • NMR spectroscopy;
  • peptides;
  • xenon

Graphical Abstract

Thumbnail image of graphical abstract

Caged in: The formation of a complex between a peptide ligand and a major histocompatibility complex (MHC) class II protein is detected by a 129Xe biosensor. Cryptophane molecules that trap Xe atoms are modified with a hemagglutinin (HA) peptide, which binds to the MHC protein. The interaction can be monitored by an NMR chemical shift change of cage–HA bound 129Xe.

Abstract

Caged in: The formation of a complex between a peptide ligand and a major histocompatibility complex (MHC) class II protein is detected by a 129Xe biosensor. Cryptophane molecules that trap Xe atoms are modified with a hemagglutinin (HA) peptide, which binds to the MHC protein. The interaction can be monitored by an NMR chemical shift change of cage–HA bound 129Xe.

View Full Article with Supporting Information (HTML) Get PDF (553K)