Communication

Mechanical Nanosensor Based on FRET within a Thermosome: Damage-Reporting Polymeric Materials†

  1. Nico Bruns Dr.1,2,
  2. Katarzyna Pustelny1,
  3. Lisa M. Bergeron Dr.1,
  4. Timothy A. Whitehead Dr.1,
  5. Douglas S. Clark Prof.1

Article first published online: 28 APR 2009

DOI: 10.1002/anie.200900554

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 48, Issue 31, pages 5666–5669, July 20, 2009

Additional Information

How to Cite

Bruns, N., Pustelny, K., Bergeron, L., Whitehead, T. and Clark, D. (2009), Mechanical Nanosensor Based on FRET within a Thermosome: Damage-Reporting Polymeric Materials. Angewandte Chemie International Edition, 48: 5666–5669. doi: 10.1002/anie.200900554

Author Information

  1. 1

    Department of Chemical Engineering, University of California, 201 Gilman Hall, Berkeley, CA 94720 (USA), Fax: (+1) 510-643-1228 http://www.cchem.berkeley.edu/clarkgrp/

  2. 2

    Current address: Dept. of Chemistry, University of Basel (Switzerland)

  1. †

    We are indebted to Prof. Jeffrey Urbach (Georgetown University) for helpful discussions and assistance in preparing the manuscript. We are grateful to Prof. Anthony R. Clarke and Dr. M. Giulia Bigotti (School of Medical Sciences, University of Bristol (UK)) for a gift of the plasmid that encodes the native THS. We thank Holly L. Aaron at the Berkeley Molecular Imaging Center for assistance with the confocal and FLIM microscopy, as well as Prof. Jean M. Fréchet and Prof. Carolyn R. Bertozzi for the use of their instrumentation. The research described in this article was funded by the Air Force Office of Scientific Research (FA9550-07-1-0116). FRET=fluoresence resonance energy transfer.

Publication History

  1. Issue published online: 15 JUL 2009
  2. Article first published online: 28 APR 2009
  3. Manuscript Received: 29 JAN 2009

Funded by

  • Air Force Office of Scientific Research. Grant Number: FA9550-07-1-0116

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Keywords:

  • FRET;
  • hybrid materials;
  • polymers;
  • proteins;
  • stress detection

Graphical Abstract

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Under stress: Changes in stress of the polymer matrix in a protein–polymer hybrid material result in changes of conformation of the protein complex, thus resulting in a damage-reporting material (see picture). The reporter is an engineered chaperonin that covalently entraps a pair of fluorescent proteins. Deformation of the chaperonin leads to a change in fluorophore distance and a change in the fluorescene resonance energy transfer (FRET) signal.

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