Non-Hydrolyzable RNA–Peptide Conjugates: A Powerful Advance in the Synthesis of Mimics for 3′-Peptidyl tRNA Termini

Authors

  • Holger Moroder,

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • Jessica Steger,

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • Dagmar Graber,

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • Katja Fauster,

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • Krista Trappl,

    1. Innsbruck Biocenter, Medical University of Innsbruck, Divison of Genomics and RNomics, 6020 Innsbruck (Austria)
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  • Viter Marquez Dr.,

    1. Gene Center, Department for Chemistry and Biochemistry and Center for Integrated Protein Science (CiPS-M), Ludwig-Maximillians-Universität, 81377 Munich (Germany)
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  • Norbert Polacek Priv.-Doz. Dr.,

    1. Innsbruck Biocenter, Medical University of Innsbruck, Divison of Genomics and RNomics, 6020 Innsbruck (Austria)
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  • Daniel N. Wilson Dr.,

    1. Gene Center, Department for Chemistry and Biochemistry and Center for Integrated Protein Science (CiPS-M), Ludwig-Maximillians-Universität, 81377 Munich (Germany)
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  • Ronald Micura Prof. Dr.

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • H. Moroder and J. Steger contributed equally to this work. Funding by the Austrian Science Foundation FWF (P17864 to R.M.; Y315 to N.P.) and the Ministry of Science and Research (GenAU project consortium “non-coding RNAs” P0726-012-012 to R.M. and D1042-011-011 to N.P) and the Deutsche Forschungsgemeinschaft DFG (WI3285/1-1 to D.N.W.) is acknowledged.

Abstract

Translation of specific small peptides on the ribosome can confer resistance to macrolide antibiotics. To reveal the molecular details of this and related phenomena, stable RNA–peptide conjugates that mimic peptidyl-tRNA would be desirable, especially for ribosome structural biology. A flexible solid-phase synthesis strategy now allows efficient access to these highly requested derivatives without restriction on the RNA and peptide sequences.

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