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Review
Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality
Article first published online: 27 AUG 2009
DOI: 10.1002/anie.200900942
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
Angewandte Chemie International Edition
Volume 48, Issue 38, pages 6974–6998, September 7, 2009
Additional Information
How to Cite
Sletten, E. and Bertozzi, C. (2009), Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality. Angewandte Chemie International Edition, 48: 6974–6998. doi: 10.1002/anie.200900942
Publication History
- Issue published online: 1 SEP 2009
- Article first published online: 27 AUG 2009
- Manuscript Revised: 21 MAY 2009
- Manuscript Received: 18 FEB 2009
- Abstract
- Article
- References
- Cited By
Keywords:
- alkynes;
- azides;
- bioconjugation;
- bioorthogonal reactions;
- Staudinger ligation
Graphical Abstract
Being specific: A combination of selective chemical transformations and methods to modify biological species has yielded new insights into cellular processes. Key to these new techniques are bioorthogonal chemical reactions, whose components must react rapidly and selectively with each other under physiological conditions in the presence of the plethora of functionality found within living systems.
Abstract
The study of biomolecules in their native environments is a challenging task because of the vast complexity of cellular systems. Technologies developed in the last few years for the selective modification of biological species in living systems have yielded new insights into cellular processes. Key to these new techniques are bioorthogonal chemical reactions, whose components must react rapidly and selectively with each other under physiological conditions in the presence of the plethora of functionality necessary to sustain life. Herein we describe the bioorthogonal chemical reactions developed to date and how they can be used to study biomolecules.