Communication

Taking Fingerprints of DNA Polymerases: Multiplex Enzyme Profiling on DNA Arrays†

  1. Ramon Kranaster Dipl.-Chem.,
  2. Andreas Marx Prof. Dr.

Article first published online: 14 MAY 2009

DOI: 10.1002/anie.200900953

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 48, Issue 25, pages 4625–4628, June 8, 2009

Additional Information

How to Cite

Kranaster, R. and Marx, A. (2009), Taking Fingerprints of DNA Polymerases: Multiplex Enzyme Profiling on DNA Arrays. Angew. Chem. Int. Ed., 48: 4625–4628. doi: 10.1002/anie.200900953

Author Information

  1. Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany), Fax: (+49) 7531-88-5140

  1. †

    We gratefully acknowledge funding by the DFG (SPP1170) and BMBF (BioChancePlus).

Publication History

  1. Issue published online: 3 JUN 2009
  2. Article first published online: 14 MAY 2009
  3. Manuscript Revised: 24 MAR 2009
  4. Manuscript Received: 18 FEB 2009

Funded by

  • DFG. Grant Number: SPP1170
  • BMBF

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Keywords:

  • directed evolution;
  • DNA damage;
  • DNA polymerases;
  • enzymes;
  • microarrays

Abstract

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A novel screening approach based on an oligonucleotide-addressing enzyme assay enables multiplexed simultaneous profiling of DNA polymerases in nanoliter volumes in terms of their different properties. This approach was used to identify enzymes with altered properties out of a library of protein mutants.

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