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Taking Fingerprints of DNA Polymerases: Multiplex Enzyme Profiling on DNA Arrays

Authors

  • Ramon Kranaster Dipl.-Chem.,

    1. Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany), Fax: (+49) 7531-88-5140
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  • Andreas Marx Prof. Dr.

    1. Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany), Fax: (+49) 7531-88-5140
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  • We gratefully acknowledge funding by the DFG (SPP1170) and BMBF (BioChancePlus).

Abstract

A novel screening approach based on an oligonucleotide-addressing enzyme assay enables multiplexed simultaneous profiling of DNA polymerases in nanoliter volumes in terms of their different properties. This approach was used to identify enzymes with altered properties out of a library of protein mutants.

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