These authors contributed equally.
A Metal–Collagen Peptide Framework for Three-Dimensional Cell Culture†
Article first published online: 15 SEP 2009
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 48, Issue 42, pages 7813–7817, October 5, 2009
How to Cite
Pires, Marcos M., Przybyla, David E. and Chmielewski, J. (2009), A Metal–Collagen Peptide Framework for Three-Dimensional Cell Culture. Angew. Chem. Int. Ed., 48: 7813–7817. doi: 10.1002/anie.200902375
We are grateful to the NSF (0848325-CHE) for support of this research, to D. Sherman for assistance with SEM, and to E. Campanella for assistance with confocal microscopy.
- Issue published online: 30 SEP 2009
- Article first published online: 15 SEP 2009
- Manuscript Revised: 8 JUL 2009
- Manuscript Received: 4 MAY 2009
- NSF. Grant Number: 0848325-CHE
- cell encapsulation;
- collagen peptides;
- metal–organic frameworks;
- three-dimensional scaffolds
It won′t overstay its welcome: Metal ions trigger the rapid assembly of a triple-helical collagen-based peptide with ligands at the ends and center into a fibrous three-dimensional mesh under physiological conditions (see picture). Importantly for applications in regenerative medicine, a mild chelating agent causes rapid disassembly of the mesh. Human endothelial cells were readily encapsulated and cultured within the collagen-peptide network.