A Metal–Collagen Peptide Framework for Three-Dimensional Cell Culture


  • We are grateful to the NSF (0848325-CHE) for support of this research, to D. Sherman for assistance with SEM, and to E. Campanella for assistance with confocal microscopy.


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It won′t overstay its welcome: Metal ions trigger the rapid assembly of a triple-helical collagen-based peptide with ligands at the ends and center into a fibrous three-dimensional mesh under physiological conditions (see picture). Importantly for applications in regenerative medicine, a mild chelating agent causes rapid disassembly of the mesh. Human endothelial cells were readily encapsulated and cultured within the collagen-peptide network.