We thank Prof. K. Yamana and Dr. M. Nakamura of University of Hyogo for fluorescence lifetime measurements. This work was supported by a Grant in Aid for Scientific Research (S) (18105006) from JSPS.
Secondary-Structure-Inducible Ligand Fluorescence Coupled with PCR†
Article first published online: 11 SEP 2009
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 48, Issue 42, pages 7822–7824, October 5, 2009
How to Cite
Takei, F., Igarashi, M., Hagihara, M., Oka, Y., Soya, Y. and Nakatani, K. (2009), Secondary-Structure-Inducible Ligand Fluorescence Coupled with PCR. Angew. Chem. Int. Ed., 48: 7822–7824. doi: 10.1002/anie.200902449
- Issue published online: 30 SEP 2009
- Article first published online: 11 SEP 2009
- Manuscript Revised: 10 JUL 2009
- Manuscript Received: 8 MAY 2009
- hairpin primer;
- polymerase chain reaction;
- SNP typing
Not second fiddle: Hairpin secondary structures at the 5′ end of the PCR primer are transformed into a double-stranded form as the PCR proceeds. A fluorescent molecule (DANP) can bind to the single cytosine bulge (C-bulge) in the hairpin structure and emit characteristic fluorescence. When the PCR primer labeled with a C-bulge hairpin tag is used in the presence of DANP, fluorescence from the DANP-C-bulge complex decreases as the PCR proceeds.