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Patterning Mammalian Cells for Modeling Three Types of Naturally Occurring Cell–Cell Interactions

Authors

  • Zhenling Chen Dr.,

    1. CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190 (China), Fax: (+86) 10-6265-6765
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    • These authors contributed equally to this work.

  • Yong Li,

    1. CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190 (China), Fax: (+86) 10-6265-6765
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    • These authors contributed equally to this work.

  • Wenwen Liu,

    1. CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190 (China), Fax: (+86) 10-6265-6765
    2. Graduate University of the Chinese Academy of Sciences Shijingshan, Yuquan Road, 19(A), Beijing 100049 (China)
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  • Dongzhou Zhang,

    1. CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190 (China), Fax: (+86) 10-6265-6765
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  • Yuyun Zhao,

    1. CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190 (China), Fax: (+86) 10-6265-6765
    2. Graduate University of the Chinese Academy of Sciences Shijingshan, Yuquan Road, 19(A), Beijing 100049 (China)
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  • Bo Yuan,

    1. CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190 (China), Fax: (+86) 10-6265-6765
    2. Graduate University of the Chinese Academy of Sciences Shijingshan, Yuquan Road, 19(A), Beijing 100049 (China)
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  • Xingyu Jiang Prof.

    1. CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190 (China), Fax: (+86) 10-6265-6765
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  • We thank Prof. Mian Long (Institute of Mechanics, CAS) and Prof. Wei Liang (Institute of Biophysics of the Chinese Academy of Science) for generously providing cells. The Human Frontier Science Program, the Chinese Academy of Sciences, the National Science Foundation of China (20890020, 90813032, and 20605006), and the Ministry of Science and Technology (2009CB930001 and 2007CB714502) provided financial support. This work was partially supported by a fellowship from Corning, Inc., China Postdoctoral Science Foundation, and K. C. Wong Education Foundation (Hong Kong).

Abstract

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Types of stripes: Microfluidic techniques are combined with surface chemistry to pattern multiple types of cells on the same substrate to simulate three types of naturally occurring cell–cell interactions. PDMS=poly(dimethylsiloxane) stamp, FN=fibronectin to promote cell adhesion, SAM=self-assembled monolayer of an applied alkanethiol that resists cell adhesion.

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