Communication

Parallel Isotope-Based Quantification of Modified tRNA Nucleosides†

  1. Tobias Brückl‡,
  2. Daniel Globisch‡,
  3. Mirko Wagner,
  4. Markus Müller Dr.,
  5. Thomas Carell Prof. Dr.

Article first published online: 11 SEP 2009

DOI: 10.1002/anie.200902740

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 48, Issue 42, pages 7932–7934, October 5, 2009

Additional Information

How to Cite

Brückl, T., Globisch, D., Wagner, M., Müller, M. and Carell, T. (2009), Parallel Isotope-Based Quantification of Modified tRNA Nucleosides. Angew. Chem. Int. Ed., 48: 7932–7934. doi: 10.1002/anie.200902740

Author Information

  1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756

  1. ‡

    These authors contributed equally to this work.

  1. †

    We thank the CiPSM Cluster, SFB 749, the Fonds of the German Chemical Industry, and Bayer-Schering for financial support.

  2. ‡

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 30 SEP 2009
  2. Article first published online: 11 SEP 2009
  3. Manuscript Received: 22 MAY 2009

Funded by

  • CiPSM Cluster
  • Fonds of the German Chemical Industry
  • Bayer-Schering

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Keywords:

  • bioanalytical methods;
  • gene translation;
  • isotope labeling;
  • modiomics;
  • tRNA
Thumbnail image of graphical abstract

Modifications make a difference: An isotope-based mass spectrometry method allows the facile and quantitative analysis of modified tRNA nucleosides in various types of cells. This method could be capable of distinguishing between individual cell lines as well as between healthy tissue and cancer cells.

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