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Parallel Isotope-Based Quantification of Modified tRNA Nucleosides

Authors

  • Tobias Brückl,

    1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756
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    • These authors contributed equally to this work.

  • Daniel Globisch,

    1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756
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    • These authors contributed equally to this work.

  • Mirko Wagner,

    1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756
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  • Markus Müller Dr.,

    1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756
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  • Thomas Carell Prof. Dr.

    1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756
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  • We thank the CiPSM Cluster, SFB 749, the Fonds of the German Chemical Industry, and Bayer-Schering for financial support.

Abstract

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Modifications make a difference: An isotope-based mass spectrometry method allows the facile and quantitative analysis of modified tRNA nucleosides in various types of cells. This method could be capable of distinguishing between individual cell lines as well as between healthy tissue and cancer cells.

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