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Protein Scaffold Engineering Towards Tunable Surface Attachment

Authors

  • Arnon Heyman,

    1. The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, and the Otto Warburg Minerva Center for Agricultural Biotechnology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100 (Israel)
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    • These authors contributed equally to this work.

  • Izhar Medalsy,

    1. Physical Chemistry Department and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904 (Israel)
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    • These authors contributed equally to this work.

  • Oron Bet Or,

    1. The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, and the Otto Warburg Minerva Center for Agricultural Biotechnology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100 (Israel)
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  • Or Dgany Dr.,

    1. The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, and the Otto Warburg Minerva Center for Agricultural Biotechnology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100 (Israel)
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  • Maya Gottlieb,

    1. Physical Chemistry Department and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904 (Israel)
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  • Danny Porath Dr.,

    1. Physical Chemistry Department and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904 (Israel)
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  • Oded Shoseyov Prof.

    1. The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, and the Otto Warburg Minerva Center for Agricultural Biotechnology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100 (Israel)
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  • We thank Dr. Igor Brodsky and Dr. Ilan Levy for assistance and discussions, and Eran Zehavy for assistance with the graphics. This research was supported in part by a grant from the BIOMEDNANO STREP project of the European Community and a Trilateral DFG grant CU 44/3-2.

Abstract

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Ringing the changes: A selective, controlled, and tunable attachment of proteins to a metal or to insulating surfaces is presented. A ring-shaped protein encapsulating SiO2-binding peptides (see picture: yellow) was engineered to expose multiple binding sites simply by changing solvent conditions, thereby creating a single monolayer without the need for surface modification.

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