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Fluorogenically Active Leucine Zipper Peptides as Tag–Probe Pairs for Protein Imaging in Living Cells

Authors

  • Hiroshi Tsutsumi Dr.,

    1. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan)
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  • Wataru Nomura Dr.,

    1. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan)
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  • Seiichiro Abe,

    1. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan)
    2. School of Biomedical Science, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan), Fax: (+81) 3-5280-8036
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  • Tomoaki Mino,

    1. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan)
    2. School of Biomedical Science, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan), Fax: (+81) 3-5280-8036
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  • Akemi Masuda,

    1. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan)
    2. School of Biomedical Science, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan), Fax: (+81) 3-5280-8036
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  • Nami Ohashi,

    1. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan)
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  • Tomohiro Tanaka,

    1. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan)
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  • Kenji Ohba Dr.,

    1. AIDS Research Center, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640 (Japan)
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  • Naoki Yamamoto Prof.,

    1. AIDS Research Center, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640 (Japan)
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  • Kazunari Akiyoshi Prof.,

    1. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan)
    2. School of Biomedical Science, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan), Fax: (+81) 3-5280-8036
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  • Hirokazu Tamamura Prof.

    1. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan)
    2. School of Biomedical Science, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 (Japan), Fax: (+81) 3-5280-8036
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  • This work was supported in part by a grant from the Naito Foundation.

Abstract

original image

All zipped up: Pairs of leucine zipper peptides with a solvatochromic fluorescent dye show fluorogenic activity through formation of a 3α-helical bundle structure. Such peptides enable the in situ fluorescence imaging of proteins in the presence of excess fluorescent probe (see picture). Imaging of membrane-bound protein is demonstrated using these zipper peptides as functional tag–probe pairs.

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