A label for six, please! A fluorescent tetrazine derivative was used to image trans-cyclooctene-modified affinity ligands on live cancer cells through a bioorthogonal cycloaddition with a reaction rate of approximately 6000±200 M−1 s−1 in serum at 37 °C (see scheme). To maximize the fluorescence signal, up to six trans-cyclooctene moieties were attached to the antibody used to pretarget cells for labeling.
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