Communication

DNA Origami as a Nanoscopic Ruler for Super-Resolution Microscopy†

  1. Christian Steinhauer2,‡,
  2. Ralf Jungmann1,‡,
  3. Thomas L. Sobey1,
  4. Friedrich C. Simmel Prof. Dr.1,
  5. Philip Tinnefeld Prof. Dr.2

Article first published online: 14 OCT 2009

DOI: 10.1002/anie.200903308

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 48, Issue 47, pages 8870–8873, November 9, 2009

Additional Information

How to Cite

Steinhauer, C., Jungmann, R., Sobey, T., Simmel, F. and Tinnefeld, P. (2009), DNA Origami as a Nanoscopic Ruler for Super-Resolution Microscopy. Angewandte Chemie International Edition, 48: 8870–8873. doi: 10.1002/anie.200903308

Author Information

  1. 1

    Physics Department E14 & Center for Nanoscience, Technische Universität München, James-Franck-Strasse 1, 85748 Garching (Germany), Fax: (+49) 89-289-13820

  2. 2

    Angewandte Physik—Biophysik & Center for Nanoscience, Ludwig-Maximilians-Universität München, Amalienstrasse 54, 80799 München (Germany), Fax: (+49) 89-2180-2050

  1. ‡

    These authors contributed equally to this work.

  1. †

    We are grateful to Rob Fee and Helene Budjarek for experimental support, and Paul Rothemund for helpful advice. This work was supported by the DFG (Inst 86/1051-1), the Biophotonics Program of the BMBF/VDI, the Nanosystems Initiative Munich, the LMU Center for Nanoscience, and the Elitenetzwerk Bayern.

Publication History

  1. Issue published online: 4 NOV 2009
  2. Article first published online: 14 OCT 2009
  3. Manuscript Received: 18 JUN 2009

Funded by

  • DFG. Grant Number: Inst 86/1051-1
  • BMBF/VDI
  • Nanosystems Initiative Munich
  • LMU Center for Nanoscience
  • Elitenetzwerk Bayern

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Keywords:

  • DNA origami;
  • fluorescence microscopy;
  • molecular ruler;
  • nanoscopic ruler;
  • super-resolution

Graphical Abstract

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Resolving the distances: Rectangular DNA origami labeled with fluorophores at specific positions has been used as a nanoscopic ruler. Super-resolution microscopy based on the subsequent localization of single molecules enables two fluorophores at a distance of about 90 nm to be optically resolved. This combination of subdiffraction imaging and DNA nanotechnology opens up new avenues for studying nanostructures and their dynamics.

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