K.G. and K.-T.H. contributed equally to this work. This work was supported by a grant from the European Research Council (ERC 201749). A fellowship from Boehringer Inglheim Fonds (to K.G.) and the French Ministry of Research and Education (K.-T.H.) is gratefully acknowledged. 2G12 was obtained through the NIH AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH; and the HIV-1 gp120 monoclonal antibody (2G12) from Dr. Hermann Katinger. We thank Laurence Oswald for her technical assistance.
DNA-Templated Homo- and Heterodimerization of Peptide Nucleic Acid Encoded Oligosaccharides that Mimick the Carbohydrate Epitope of HIV†
Article first published online: 22 SEP 2009
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 48, Issue 41, pages 7695–7700, September 28, 2009
How to Cite
Gorska, K., Huang, K.-T., Chaloin, O. and Winssinger, N. (2009), DNA-Templated Homo- and Heterodimerization of Peptide Nucleic Acid Encoded Oligosaccharides that Mimick the Carbohydrate Epitope of HIV. Angew. Chem. Int. Ed., 48: 7695–7700. doi: 10.1002/anie.200903328
- Issue published online: 23 SEP 2009
- Article first published online: 22 SEP 2009
- Manuscript Received: 19 JUN 2009
- European Research Council. Grant Number: ERC 201749
- French Ministry of Research and Education
- bioorganic chemistry;
- peptide nucleic acids
All under control: The programmability of hybridization has been utilized to generate a combinatorial library of structures that emulate the topologies of complex carbohydrates interacting with an antibody that shows broad-spectrum activity against HIV. This simple method involves attaching oligosaccharides tagged with peptide nucleic acids onto DNA templates in a controlled manner (see schematic picture).