DNA-Templated Homo- and Heterodimerization of Peptide Nucleic Acid Encoded Oligosaccharides that Mimick the Carbohydrate Epitope of HIV


  • K.G. and K.-T.H. contributed equally to this work. This work was supported by a grant from the European Research Council (ERC 201749). A fellowship from Boehringer Inglheim Fonds (to K.G.) and the French Ministry of Research and Education (K.-T.H.) is gratefully acknowledged. 2G12 was obtained through the NIH AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH; and the HIV-1 gp120 monoclonal antibody (2G12) from Dr. Hermann Katinger. We thank Laurence Oswald for her technical assistance.


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All under control: The programmability of hybridization has been utilized to generate a combinatorial library of structures that emulate the topologies of complex carbohydrates interacting with an antibody that shows broad-spectrum activity against HIV. This simple method involves attaching oligosaccharides tagged with peptide nucleic acids onto DNA templates in a controlled manner (see schematic picture).