Infrared Photoactivation Reduces Peptide Folding and Hydrogen-Atom Migration following ETD Tandem Mass Spectrometry


  • This research was supported by the NIH (RO1 GM080148 to J.J.C.) and the NSF (CHE-0747990 to J.J.C. and CHE-0718320 to J.S.B.). ETD=electron transfer dissociation.


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Peptides see the light: The photoactivation of peptide precursor cations during electron transfer dissociation (ETD) led to the generation of isotopic cluster peaks that more closely resembled theoretically predicted product-ion distributions. This method should enable the application of ETD to low-charge-density peptide precursors, the gas-phase secondary structure of which prevents the direct formation of c- and z.-type fragment ions.