This research was supported by the NIH (RO1 GM080148 to J.J.C.) and the NSF (CHE-0747990 to J.J.C. and CHE-0718320 to J.S.B.). ETD=electron transfer dissociation.
Infrared Photoactivation Reduces Peptide Folding and Hydrogen-Atom Migration following ETD Tandem Mass Spectrometry†
Article first published online: 30 SEP 2009
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 48, Issue 45, pages 8526–8528, October 26, 2009
How to Cite
Ledvina, Aaron R., McAlister, Graeme C., Gardner, Myles W., Smith, Suncerae I., Madsen, James A., Schwartz, Jae C., Stafford, George C., Syka, John E. P., Brodbelt, Jennifer S. and Coon, Joshua J. (2009), Infrared Photoactivation Reduces Peptide Folding and Hydrogen-Atom Migration following ETD Tandem Mass Spectrometry. Angew. Chem. Int. Ed., 48: 8526–8528. doi: 10.1002/anie.200903557
- Issue published online: 20 OCT 2009
- Article first published online: 30 SEP 2009
- Manuscript Received: 30 JUN 2009
- NIH. Grant Number: RO1 GM080148
- NSF. Grant Numbers: CHE-0747990, CHE-0718320
- ionization of gases;
- mass spectrometry;
Peptides see the light: The photoactivation of peptide precursor cations during electron transfer dissociation (ETD) led to the generation of isotopic cluster peaks that more closely resembled theoretically predicted product-ion distributions. This method should enable the application of ETD to low-charge-density peptide precursors, the gas-phase secondary structure of which prevents the direct formation of c- and z.-type fragment ions.