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Infrared Photoactivation Reduces Peptide Folding and Hydrogen-Atom Migration following ETD Tandem Mass Spectrometry†

  1. Aaron R. Ledvina1,
  2. Graeme C. McAlister1,
  3. Myles W. Gardner2,
  4. Suncerae I. Smith2,
  5. James A. Madsen2,
  6. Jae C. Schwartz3,
  7. George C. Stafford Jr.3,
  8. John E. P. Syka3,
  9. Jennifer S. Brodbelt Dr.2,
  10. Joshua J. Coon Dr.1

Article first published online: 30 SEP 2009

DOI: 10.1002/anie.200903557

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 48, Issue 45, pages 8526–8528, October 26, 2009

Additional Information

How to Cite

Ledvina, Aaron R., McAlister, Graeme C., Gardner, Myles W., Smith, Suncerae I., Madsen, James A., Schwartz, Jae C., Stafford, George C., Syka, John E. P., Brodbelt, Jennifer S. and Coon, Joshua J. (2009), Infrared Photoactivation Reduces Peptide Folding and Hydrogen-Atom Migration following ETD Tandem Mass Spectrometry. Angew. Chem. Int. Ed., 48: 8526–8528. doi: 10.1002/anie.200903557

Author Information

  1. 1

    Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706-1322 (USA), Fax: (+1) 608-262-0453

  2. 2

    Department of Chemistry, University of Texas-Austin, Austin, TX 78712-1167 (USA)

  3. 3

    Thermo Fisher Scientific, San Jose, CA 95134 (USA)

  1. †

    This research was supported by the NIH (RO1 GM080148 to J.J.C.) and the NSF (CHE-0747990 to J.J.C. and CHE-0718320 to J.S.B.). ETD=electron transfer dissociation.

Publication History

  1. Issue published online: 20 OCT 2009
  2. Article first published online: 30 SEP 2009
  3. Manuscript Received: 30 JUN 2009

Funded by

  • NIH. Grant Number: RO1 GM080148
  • NSF. Grant Numbers: CHE-0747990, CHE-0718320

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Keywords:

  • ionization of gases;
  • mass spectrometry;
  • peptides;
  • photoactivation;
  • proteomics
Thumbnail image of graphical abstract

Peptides see the light: The photoactivation of peptide precursor cations during electron transfer dissociation (ETD) led to the generation of isotopic cluster peaks that more closely resembled theoretically predicted product-ion distributions. This method should enable the application of ETD to low-charge-density peptide precursors, the gas-phase secondary structure of which prevents the direct formation of c- and z.-type fragment ions.

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