This work was supported by the Swiss National Science Foundation (grant no. 116868), the Dr. Helmut Legerlotz Stiftung, and the University of Zürich. We thank the University of Zürich Center for Microscopy and the Institute of Molecular Cancer Research, as well as Giulio Fiaschetti and Prof. Alexandre Arcaro for technical assistance.
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Communication
Guanidinium-Modified Phthalocyanines as High-Affinity G-Quadruplex Fluorescent Probes and Transcriptional Regulators†
Article first published online: 30 OCT 2009
DOI: 10.1002/anie.200903685
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
Angewandte Chemie International Edition
Volume 48, Issue 49, pages 9362–9365, November 23, 2009
Additional Information
How to Cite
Alzeer, J., Vummidi, B., Roth, P. and Luedtke, N. (2009), Guanidinium-Modified Phthalocyanines as High-Affinity G-Quadruplex Fluorescent Probes and Transcriptional Regulators. Angewandte Chemie International Edition, 48: 9362–9365. doi: 10.1002/anie.200903685
- †
Publication History
- Issue published online: 17 NOV 2009
- Article first published online: 30 OCT 2009
- Manuscript Received: 6 JUL 2009
Funded by
- Swiss National Science Foundation. Grant Number: 116868
- Dr. Helmut Legerlotz Stiftung
- University of Zürich
Keywords:
- DNA;
- fluorescent probes;
- G-quadruplexes;
- polymerase chain reaction;
- RNA
Graphical Abstract
Fluorescence is ON and transcriptional control is OFF when the guanidinium-modified zinc phthalocyanine Zn-DIGP binds a G-quadruplex DNA from the c-Myc promoter. With an equilibrium dissociation constant Kd of less than 2 nM, this interaction is the strongest binding interaction between a G-quadruplex structure and a small molecule reported to date.