This research was supported by Grant-in-Aid number 19655063 from the Ministry of Education, Science, Sports, and Culture (Japan). RNAi=RNA interference.
1521-3773/asset/2002_left.gif?v=1&s=ac6b0d94a94d7ce7a210002b8096b42feffc0bcf)
1521-3773/asset/2002_right.gif?v=1&s=451042aa3415ae3ad0729984d26dee1866aca82e)
Communication
An Activatable siRNA Probe: Trigger-RNA-Dependent Activation of RNAi Function†
Article first published online: 10 NOV 2009
DOI: 10.1002/anie.200903925
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Masu, H., Narita, A., Tokunaga, T., Ohashi, M., Aoyama, Y. and Sando, S. (2009), An Activatable siRNA Probe: Trigger-RNA-Dependent Activation of RNAi Function. Angew. Chem. Int. Ed., 48: 9481–9483. doi: 10.1002/anie.200903925
- †
Publication History
- Issue published online: 30 NOV 2009
- Article first published online: 10 NOV 2009
- Manuscript Revised: 10 OCT 2009
- Manuscript Received: 17 JUL 2009
Funded by
- Ministry of Education, Science, Sports, and Culture (Japan)
Keywords:
- activatable probes;
- hairpin structure;
- RNA interference;
- RNA recognition;
- small interfering RNA
Catch and release: A hairpin-shaped sense strand (red)/antisense strand (blue) siRNA probe was designed to be activated by a trigger RNA molecule. In the presence of the trigger RNA, clear OFF-to-ON switching of RNAi activity was observed with high sequence dependency (see schematic illustration).